Blood is the most frequent type of evidence used in criminal cases, commonly used to prove the modus operandi, actus reus, and corpus delicti of a crime. The initial step in a forensic investigation of blood is the presumptive inspection of likely blood stains. Presumptive tests are typically performed at the crime site and serve as the basis for further investigation. Testing with benzidine is a common presumptive technique used for detecting blood. Since Criminals frequently use a variety of techniques in order to cover up or entirely remove blood stains from their clothing or other materials. Therefore, this study aims to investigate the effectiveness of the Benzidine Test in identifying dried bloodstains on different types of fabrics after multiple washing cycles and also determines how detergents and washing techniques affect the fabric's ability to retain bloodstains.