<p>For the quantitative measurement of ganciclovir in pharmaceutical formulations, a straightforward, sensitive, and trustworthy spectrophotometric technique was created.&nbsp; Ganciclovir is oxidised with an excess of N-Bromo succinimide in acidic media, and the unreacted NBS is then determined using the methylene blue bleaching method at 610 nm.&nbsp; With a good correlation coefficient (R2 = 0.9997), the decline in dye intensity exhibited a linear relationship with GCV content within the range of 1&ndash;35 &micro;g/mL.&nbsp; With Sandell&#39;s sensitivity of 0.169 &micro;g&middot;cm⁻&sup2; and a molar absorptivity of 3.39 &times; 10&sup3; L&middot;mol⁻&sup1;&middot;cm⁻&sup1;, the method demonstrated good sensitivity.&nbsp; Excellent recovery values (99&ndash;99.8%) and low %RSD (&lt;1.2%) were confirmed by accuracy precision studies.&nbsp; The measurement was not affected by common excipients.&nbsp; For routine analysis of ganciclovir in pharmaceutical products, the suggested approach proved effective.</p>