<p>India&rsquo;s goal of becoming a developed nation by 2047 emphasizes the need of long-term socioeconomic growth, which is backed by scientific research and ecologically responsible government. Urban freshwater ecosystems are critical for ecological balance, public health, and economic sustainability; yet, rapid urbanization and increased human activity have caused significant damage. The current study examines the utility of zooplankton communities as biological indicators for assessing urban water quality and ecosystem health, utilizing Amanishah Nala in Jaipur (Rajasthan, India) as a case study. Seasonal sampling was conducted at chosen upstream, contaminated, and downstream sites to investigate geographical and temporal differences. Zooplankton diversity, abundance, and community organization were investigated, together with essential water physicochemical parameters. Species identification was done with standard taxonomic keys, and ecological status was assessed with biodiversity indicators such the Shannon-Wiener Index. Statistical studies were used to investigate the links between zooplankton assemblages and environmental factors. The study is designed to provide baseline data on the biological state of the urban freshwater system and establish the efficacy of zooplankton as water quality indicators. The findings may promote science-based urban water management plans and highlight the necessity of including biological indicators into environmental monitoring programs to align with India&rsquo;s sustainable development goals.</p>

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